Prevalence and molecular study of moniezia species isolate from cattle in Wasit Province, Iraq

Abstract

Moniezia spp. parasitize the intestines of ruminants causing Monieziasis, M. expansa and M. benedeni (Cyclophyllidea: Anoplocephalidae) are large species of tapeworm that occurs in sheep and cattle and inhabits the small intestine.The current study was conducted during the period from December 2020 to August 2021 for detect the infection of Moniezia parasite in cattle using the traditional and molecular diagnostic methods as well as for confirmation of Moniezia spp. in cattle by the phylogenetic analysis. The samples fresh adult worms Moniezia were collected from intestines of cattle the locations of samples were obtained from abattoirs in Wasit province and also regarding months, sexes and ages affecting on infection prevalence rate.Results of microscopically, the samples fresh adult worms Moniezia spp. were collected from intestines of 125 cattle and stained with semichon's acid carmine and examined by light microscope, the results were revealed on 6 (4.8%) positive. Concerning to Moniezia spp. have been identified and classified out of 6 from cattle showed that M. expansa and M. benedeni 3 (50%) and 3(50%)respectively, without a significant difference.