Cultivation of Oyster Mushroom (Pleurotus ostreatus) On Substrate Composed from Corn Stover Supplemented with Cotton Seed Waste in Ambo University

Abstract

Mushroom cultivation is one of the advancing microbial biotechnologies for supply of nutrition, medicinal application and for recycling of long lignocelluloses organic wastes. The main aim of this study was to assess the usability of corn Stover for the production of oyster mushroom (Pleurotus ostreatus) from December 2017 to March 1018 at mushroom house and biology laboratory of Ambo University. The mushroom culture was maintained on potatoes dextrose agar, and the spawn was prepared on yellow sorghum grain and substrate was collected from Ambo Woreda Kera kebele and processed, sterilized and inoculated with 10% of the spawn. There were ten different treatments (T1-T10) three triplication the major substrates. From all the different treatments T3 showed the fastest mycelia extension (0.35 cm/day) and T9 and T10 showed slowest mycelia extension (0.23and 0.21 cm/day) respectively. Treatment 1(T1), T2 and T3 showed shortest incubation periods (83days) and T10 had longer (95 days) for overall production. Treatment 4 showed shortest mean periods from pinning to maturation in the 2nd, 3rd and 4th harvests (8 to 5 days), while T1 took longer incubation periods 10 to 8 days in all the four harvests. Treatment 3 showed highest fresh weights in 1st flush (1000g) and T5 gave least fresh weight (150g). Maximum number (6cm) of bunches was recorded from T6 and the least from T1, T3, T4, T8, T9 and T10 equally (4cm). Pilus diameter was maximum from T10 (19.5 cm) and the (7cm) was noticed from T2, T6 and T7 of corn. The longest stipe length (6cm) recorded from T10 and shortest stipe length 2cm recorded from T8. Highest number of abort was recorded from T3 (24) and the lowest from T10 (1cm). The highest total fresh weight of fruit body and biological efficiency were recorded in T2, and T3 (2021– 2181g) respectively and the least from T10 (1281g). The highest Spent Oyster Mushroom (SOMS) 76.07% was recorded from T1 and lowest (54.1%).