A Simple and Effective "elimination" Approach for Selective Cancer Therapy to Reveal the Role of H2O2

Abstract

Fluorescence imaging capability for visualizing tumor microenvironments can play a role in advancing drug discovery efforts, exploring therapeutic efficacy, and thus significantly improving the prognosis. Specifically, we present the design, synthesis, spectroscopic properties, and the targeting diagnosis of a single boronate-Appended benzorhodol. Drug release from the prodrug LHX-B-CPT was detected by tackling the fluorescent signal after the addition of H2O2 to the target cancer cells. The prodrug entered the cells via endocytosis mechanisms due to the folate unit, highlighting that the final delivery locations can minutely influence drug efficacy. Thus, this theranostic system is a new therapeutic agent, based on the reasoning that the depletion of H2O2 would be readily detected at the subcellular level by the fluorescence changes.