Secretion of proteins and antibody fragments from transiently transfected endothelial progenitor cells

Abstract

In neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, multiple sclerosis and amyotrophic lateral sclerosis, neuroinflammation can lead to blood-brain barrier (BBB) breakdown. After intravenous or intra-arterial injection into mice, endothelial progenitor cells (EPCs) home to the damaged BBB to promote neurovascular repair. Autologous EPCs transfected to express specific therapeutic proteins offer an innovative therapeutic option. Here, we demonstrate that EPC transfection by electroporation with plasmids encoding the reporter protein GFP or an anti-β-amyloid antibody fragment (Fab) leads to secretion of each protein. We also demonstrate the secreted anti-β-amyloid Fab protein functions in β-amyloid aggregate solubilization.