Association of methylenetetrahydrofolate reductase gene C677T polymorphism with multiple sclerosis in Turkish patients

Abstract

Background and Aim: Multiple sclerosis (MS) is a chronic neurodegenerative autoimmune disease of the central nervous system. Genetic risk factors are known to contribute to the etiology of MS. Methylenetetrahydrofolate reductase (MTHFR) gene C677T polymorphism has been associated with susceptibility to various autoimmune diseases. The aim of this study was to investigate a possible association between the MTHFR gene C677T polymorphism and MS in Turkish patients. Methods: The study included 130 MS patients and 150 groupmatched controls. Genomic DNA was isolated and genotyped using polymerase chain reactionYbased restriction fragment length polymorphism assay for the MTHFR gene exon C677T polymorphism. Results: The genotype and allele frequencies of C677T polymorphism showed statistically significant differences between MS patients and controls (P = 0.002 and P = 0.002; odds ratio, 1.79; 95% confidence interval, 1.23-2.63, respectively). A significant association was observed when the patients were compared with the controls according to CC genotype versus CT + TT genotypes (P = 0.0005; odds ratio, 2.35; 95% confidence interval, 1.45-3.82). There were no statistically significant association between MTHFR gene C677T polymorphism and baseline clinical and demographical characteristics of MS patients. Conclusions: These results showed that T allele of C677T polymorphism was associated with MS susceptibility in Turkish population.