Reallocation of nitrogen and phosphorus from roots drives regrowth of grasses and sedges after defoliation under deficit irrigation and nitrogen enrichment

Abstract

Reallocation of nutrients from roots to shoots is essential for plant regrowth in grasslands, particularly in nutrient-poor conditions. However, the response of root nutrient reallocation to changes in nitrogen (N) and water availability remains largely unknown. Using a novel 15N and 32P labelling technique, we quantified the contribution of N and phosphorus (P) to shoot regrowth from either root reallocation or direct soil uptake for perennial grasses exposed to high-frequency deficit irrigation (HFDI) and N addition. Without N addition, HFDI showed no impact on uptake and reallocation of N and P, likely due to unaffected soil N availability and a greater diffusion barrier offsetting increased accumulation in plant-available soil P. With N addition, HFDI increased plant N rather than P uptake, because of increasing soil N availability instead of P under combined HFDI and N addition. The HFDI decreased both N and P reallocation with N addition, possibly due to exhaustion of nutrient reserves in roots that were re-allocated above-ground. Reallocation contributed 48%–97% of N and 58%–79% of P required during the first 2 weeks of shoot regrowth. Synthesis. Our results highlight the importance of N and P reallocation from roots to buffer against changes in soil N and P availability and to maintain N:P ratio in shoot regrowth.