Larval age and nutrition affect the susceptibility of Culex quinquefasciatus (Diptera: Culicidae) to temephos

Abstract

Larval age and nutrition significantly affected the insect’s physiology.These influences are important when rearing a population of vectors that is used to monitor the resistance level, in which standardized conditions are crucial for a more harmonized result. Little information has been reported on the effects of larval age and nutrition on the susceptibility of insects to insecticides, and therefore, we studied the effects on the susceptibility of Culex quinquefasciatusSay’s (Diptera: Culicidae) larvae to temephos by comparing the median lethal concentration (LC50) after 24 hr between the second and fourth instar larvae and between the larvae that fed on protein-based and carbohydrate-based larval diets.The susceptibility of the larvae was significantly affected by the larval diets, as the larvae that fed on protein-based beef food and milk food demonstrated significantly higher LC50 value compared with the larvae that fed on carbohydrate-based food: lab food and yeast food.The larval diet interacted significantly with the larval age: while the second instar larvae were susceptible to temephos when supplied with carbohydrate-based food, the second and fourth instar larvae had no significant effect when supplied with protein-based diets, implying that a protein-rich environment may cause the mosquito to be less susceptible to temephos.This study suggested the importance of standardizing nutrition when rearing a vector population in order to obtain more harmonized dosage–response results in an insecticide resistance monitoring program. Future research could focus on the biochemical mechanism between the nutrition and the enzymatic activities of the vector.