Transgene design.

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Abstract

Transgenics are powerful mouse models to understand the biological functions of genes. This chapter gives a short overview of the requirements and considerations in designing a transgene. In addition, potential important choices that have to be made in advance for the successful designing and generating a transgenic mouse model are discussed. Methods for DNA purification for microinjection are also provided in this chapter.

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van de Sluis, B., & Voncken, J. W. (2011). Transgene design. Methods in Molecular Biology (Clifton, N.J.), 693, 89–101. https://doi.org/10.1007/978-1-60761-974-1_6

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