Biopolymer coated coreshell magnetite nanoparticles for rifampicin delivery

Abstract

In this study, chemically synthesized magnetite was produced where the size was found to be size between 50 and 60nm with para ferro magnetic property. Coreshell magnetite nanoparticles was produced with initial coating of rifampicin by ultrasonication and was encapsulated with any of one among four different biopolymers namely chitosan, starch, casein and polyhydroxybutyrate (PHB). Coreshell nanoparticles were analysed for their drug loading efficiency and drug release studies. PHB and chitosan loaded particles were observed to release drug steadily. All the biopolymer coated nanoparticles were subjected to antibacterial activity against Klebsiella sp by agar well diffusion method. PHB and chitosan coating of the particles were found to be the best for holding rifampicin. Magnetite was subjected for MTT based cytotoxicity assay against peripheral mononuclear cells and its LC50 was found at 60 μg/ml.