Effect of Protected Corn Oil Supplementation Ratio on Ruminal Fatty Acids Profile

Abstract

The study was designed to evaluate the effect of non-and protected corn oil supplementation in the series of ratios on the in-vitro ruminal fatty acid profile. Corn oil was protected by blending skim milk powder and corn oil (2:1) and adding formaldehyde at a concentration of 1,5%. The substrate as basal diet consisted of Elephant grass (Pennisetum purpureum) and wheat bran, with the ratio 60%:40%-based on dry matter. The feed substrate was supplemented with non-and protected corn oil (PCO) following different combination ratio treatments: T0: 0% and 0% (unsupplemented group), T1: 5% and 0%, T2: 3.75% and 1.25%, T3: 2.5% and 2.5%, T4: 1.25% and 3.75%, T5: 0% and 5%. The effects of these substances on the ruminal fatty acid profile were investigated using in vitro ruminal fermentation method (Tilley and Terry, 1963). The findings showed that T1, T2, and T3 significantly (P<0.01) increased stearic acid (18:0) and vaccenic acid (trans-11, C18:1).