Fusarin C production by Fusarium spp. from Spain

Abstract

From 657 samples of grains and feedstuffs (1991 to 1992 and 1992 to 1993 surveys), collected in two different geographical areas of Spain, 154 isolates of Fusarium spp. were obtained. The isolates were screened for their ability to produce fusarin C in a modified 10% ICI N synthetic liquid medium. The production of fusarin C was verified by means of spectrophotometry, thin layer chromatography, high-performance liquid chromatography, and nuclear magnetic resonance. Results showed that more than 57% of tested isolates were able to produce fusarin C at levels of 0.04 to 200 μg/liter of ICI medium. There were no statistically significant differences (P < 0.05) between levels of fusarin C production and the geographical origin of the Fusarium isolates. The Fusarium isolates that produced fusarin C were identified as E moniliforme, F. oxysporum, F. sporotrichioides, and E poae. This is the first report of fusarin C production by F. oxysporum.