Antioxidant activity of biopigment fractions from golden apple snail eggs (Pomacea canaliculata)

Abstract

Biopigment is a natural non-toxic, renewable and environmental friendly dye. The golden apple snail eggs is known to contain carotenoid groups such as astaxanthin pigment. The purpose of this study was to determine the antioxidant activity of biopigment from acetone and methanol fractions with various in vitro test. Biopigment extracted with acetone had the highest antioxidant activity with IC50 DPPH method of 130.52±3.07 μg/mL and ABTS of 125.89±1.70 μg/mL. The result of total antioxidant capacity with FRAP method of 184.11±3.92 μmol Fe2+/g extract and CUPRAC method of 29.17±3.58 μg/mL ascorbic acid/g extract. The second fraction from acetone solvent was the best fraction with IC50 DPPH method of 92.08±2.85 μg/mL and ABTS of 144.80±3.33 μg/mL. The result of total antioxidant capacity with FRAP method of 259.11±3.93 μmol ascorbic acid g-1 extract. Identification with LC-MS/MS on the second fraction found astaxanthin pigment and five other pigments.