Germinação e crescimento in vitro de Epidendrum secundum Jacq. (Orchidaceae) em diferentes meios de cultivo e períodos de exposição a agentes desinfestantes seminais

Abstract

In vitro germination and growth of Epidendrum secundum Jacq. (Orchidaceae) in different culture media and periods of exposition to seminal disinfectant agents. Epidendrum secundum is an orchid commercialized and used for ornamental purposes; thus in vitro culture is highly important, since it is fast, low cost and has high productivity. This work evaluated the in vitro germination and growth of E. secundum, derived from self pollination in 3 distinct culture media: MS formulated with half macronutrient concentration, Kyoto medium and a medium formulated with Peters ® fertilizers, a commercial fertilizer (NPK 09-45-15) at 2 g.L-1. The seeds were exposed to calcium hypochlorite (5%), sodium hypochlorite (5%) for disinfection over 2.5; 5; 7.5 and 10 minutes. At the end of the experiment, we found that the interaction between the use of calcium hypochlorite (5%) as a disinfectant, in 5 and 10 min. exposure time, with MS 1/2 macronutrients are the most suitable for the in vitro cultivation of this species.