LINKAGE RELATIONSHIPS OF NINE ENZYME LOCI IN SUNFISHES (LEPOMIS; CENTRARCHIDAE)

Abstract

Linkage relationships of nine enzyme loci; aconitase (Acon), esterase (Est), glucosephosphate isomerase A and B (Gpi), glycerate-2-dehydrogenase (G2dh), malic enzyme (Me), phosphoglycerate kinase (Pgk), phosphoglucomutase (Pgm) and superoxide dismutase (Sod), were investigated in sunfishes (Lepomis, Centrarchidae). Reciprocal F1 hybrids produced from crosses between green sunfish (Lepomis cyanellus) and redear sunfish (L. microlophus) were backcrossed with each of the two parental species. A three-point linkage map comprising G2dh, Pgk and Sod is reported. The frequencies of recombination between G2dh and Pgk and between Pgk and Sod are estimated as 45.3 and 24.7%. The remaining six loci assort independently. Possible linkage conservation and homology of this linkage group with those of other vertebrate species are discussed.