A non-barbital buffer for immunoelectrophoresis and zone electrophoresis in agarose gels

Abstract

The authors describe buffer for both immunoelectrophoresis and zone electrophoresis procedures in agarose gels. Tricine (N-tris(hydroxymethyl)methylglycine] and Tris [2-amino-2-(hydroxymethyl)-1,3-propanediol] are the main components. This buffer eliminates the procurement cost and inventory control associated with formulation of barbital buffers. The authors compare it with other common buffer formulations.