Activity of isolated staphylococcal bacteriophage in treatment of experimentally induced chronic osteomyelitis in rabbits

Abstract

The central goal of this research was to explore the use of Staphylococcus aureus specific-bacteriophage as an alternative to antibiotics in treatment of chronic osteomyelitis in rabbits by inoculation of methicillin resistant Staphylococcus aureus (MRSA) in tibia. The current study included isolation of bacteriophage from swage water by using agar overlay method. While the second experiment was evaluation of phage activity in treatment of chronic osteomyelitis in rabbits in comparison with ceftaroline. Results of our research showed that all animals of infected groups with MRSA before treatment exhibited clinical signs of osteomyelitis after 21 days of inducing infection. Group C animals that dosed with 3 × 108 pfu/ml of Staph-specific bacteriophage for two weeks intramuscularly showed faster recovery than group D that treated with 40 mg/kg B.W of ceftaroline for two weeks intramuscularly. We came in concluded that a bacteriophage was a good choice for treatment of a multiple drug resistant pathogen such as Staphylococcus aureus in shorter time with minimal side effects.