Antioxidant activity in seeds of maize genotypes with different percentage of exotic germplasm

Abstract

In order to broaden the genetic base of maize (Zea mays L.) germplasm, it is necessary to integrate exotic materials into adapted breeding materials. The aim of the study was to compare antioxidative systems of two adapted maize inbred lines (A and B) with exotic germplasm, Drought Tolerant Population (DTP), and their backcrosses with DTP (A1, A2 and B1, B2). The content of low-molecular weight antioxidants, proline and phenolics, as well as antioxidant capacity, detected as free radical scavenging activities against DPPH radical, were measured in maize seeds. Proline content in both, embryo and endosperm was higher in backcrosses than in inbred lines and DTP, and increased in embryo by getting higher percentage of exotic germplasm. Contrary, phenolic content and DPPH radical scavenging activity of seeds, which were higher in adapted inbred lines than in DTP, were slightly decreased in their backcrosses with DTP.U cilju prosirenja geneticke osnove germplazme kukuruza (Zea mays L.), neophodna je inkorporacija egzoticnog u lokalni adaptirani selekcioni materijal. U radu su uporedjeni antioksidativni sistemi dve lokalne inbred linije (A i B) sa antioksidativnim sistemima egzoticne germplazme (DTP) i povratnih ukrstanja tih inbred linija i DTP-a (A1, A2, B1 i B2). U semenu kukuruza meren je sadrzaj antioksidanata male molekulske tezine, prolina i ukupnih fenola, kao i antioksidativni kapacitet, izrazen preko sposobnosti hvatanja slobodnih DPPH radikala. Rezultati su pokazali porast nivoa prolina, kako u klici, tako i u endospermu, kod A1, A2, B1 i B2 genotipova, u odnosu na ciste inbred linije i DTP. Takodje, nivo prolina se povecavao sa povecanjem udela egzoticne germplazme. Nasuprot tome, kod cistih inbred linija je bio veci nivo ukupnih fenola i stepen hvatanja slobodnih DPPH radikala nego kod DTP-a, i sa tendencijom neznatnog smanjenja kod A1, A2, B1 i B2 genotipova.