Physical mapping of ribosomal DNA genes on Jatropha curcas chromosomes by multicolor FISH

Abstract

Jatropha curcas L. belonging to the Euphorbiaceae is becoming a significant crop in tropical areas nowadays. The genetic diversity among 1 African and 5 Asian varieties were analyzed by multicolor fluorescence in situ hybridization (McFISH) using 5S and 45S ribosomal RNA genes (rDNAs). One locus of 5S rDNA and 2 loci for 45S rDNA were detected at specific regions of the chromosomes in all the materials. Also, telomeric repeats (TTTAGGG)n were localized on the terminal regions of all chromosomes. The present results confirm the stability of these major repeated sequences among Jatropha lines. © 2009 The Japan Mendel Society.