The N-acetyl-d-glucosamine repressor NagC of Vibrio fischeri facilitates colonization of Euprymna scolopes

Abstract

To successfully colonize and persist within a host niche, bacteria must properly regulate their gene expression profiles. The marine bacterium Vibrio fischeri establishes a mutualistic symbiosis within the light organ of the Hawaiian squid, Euprymna scolopes. Here, we show that the repressor NagC of V.fischeri directly regulates several chitin- and N-acetyl-d-glucosamine-utilization genes that are co-regulated during productive symbiosis. We also demonstrate that repression by NagC is relieved in the presence of N-acetyl-d-glucosamine-6-phosphate, the intracellular form of N-acetyl-d-glucosamine. We find that gene repression by NagC is critical for efficient colonization of E.scolopes. Further, our study shows that NagC regulates genes that affect the normal dynamics of host colonization. © 2011 Blackwell Publishing Ltd.