Decline of influenza-specific CD8+T cell repertoire in healthy geriatric donors

15Citations
Citations of this article
22Readers
Mendeley users who have this article in their library.

Abstract

Background: While influenza vaccination results in protective antibodies against primary infections, clearance of infection is primarily mediated through CD8+T cells. Studying the CD8+T cell response to influenza epitopes is crucial in understanding the disease associated morbidity and mortality especially in at risk populations such as the elderly. We compared the CD8+T cell response to immunodominant and subdominant influenza epitopes in HLA-A2+control, adult donors, aged 21-42, and in geriatric donors, aged 65 and older.Results: We used a novel artificial Antigen Presenting Cell (aAPC) based stimulation assay to reveal responses that could not be detected by enzyme-linked immunosorbent spot (ELISpot). 14 younger control donors and 12 geriatric donors were enrolled in this study. The mean number of influenza-specific subdominant epitopes per control donor detected by ELISpot was only 1.4 while the mean detected by aAPC assay was 3.3 (p = 0.0096). Using the aAPC assay, 92% of the control donors responded to at least one subdominant epitopes, while 71% of control donors responded to more than one subdominant influenza-specific response. 66% of geriatric donors lacked a subdominant influenza-specific response and 33% of geriatric donors responded to only 1 subdominant epitope. The difference in subdominant response between age groups is statistically significant (p = 0.0003).Conclusion: Geriatric donors lacked the broad, multi-specific response to subdominant epitopes seen in the control donors. Thus, we conclude that aging leads to a decrease in the subdominant influenza-specific CTL responses which may contribute to the increased morbidity and mortality in older individuals. © 2011 Lee et al; licensee BioMed Central Ltd.

Cite

CITATION STYLE

APA

Lee, J. B., Oelke, M., Ramachandra, L., Canaday, D. H., & Schneck, J. P. (2011). Decline of influenza-specific CD8+T cell repertoire in healthy geriatric donors. Immunity and Ageing, 8. https://doi.org/10.1186/1742-4933-8-6

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free