Deschampsia cespitosa , a member of the Poaceae family, is a self-incompatible, tussock-forming perennial grass. Genetic material was collected from nine populations of D. cespitosa growing in three regions of Northern Ontario exhibiting different levels of metal contamination in the soil. Analysis by Inter-simple sequence repeat (ISSR) revealed a mean level of polymorphic loci of 46%, 74% and 69% in the Cobalt, Sudbury and Manitoulin Island regions, respectively. The between-populations variance contributed only 13.6% of the total molecular variance while the within-populations variance accounted for 71.2%. The genetic distance among the D. cespitosa populations ranged from 0.059 to 0.488. No single locus appeared to be specific to contaminated sites. Cross-species amplification of several nuclear microsatellite primer pairs identified five polymorphic microsatellite loci in D. cespitosa . Microsatellite analysis revealed a mean number of alleles per population across loci ranging from 3 in Little Current to 5.2 in Cobalt-3. The observed and expected heterozygosities varied from 0.413 and 0.48 in the Mississagi Lighthouse population to 0.645 to 0.76 in the Cobalt-3 population. The mean degree of population differentiation was found to be 19.4%. The dendrogram constructed based on ISSR and microsatellite data both reveal the clustering of the three Cobalt populations and the clustering of the four Sudbury populations. Overall the results of the present study indicate no apparent correlation between the level of genetic diversity and metal contamination levels in the soil for the D. cespitosa populations in Northern Ontario.
Gervais, S., & Nkongolo, K. (2011). Effect of Metal Contamination on the Genetic Diversity of Deschampsia cespitosa Populations from Northern Ontario: An Application of ISSR and Microsatellite Markers. In Relevant Perspectives in Global Environmental Change. InTech. https://doi.org/10.5772/31164