Isolation, purification and characterization of active compound from Andrographis paniculata L. and testing its anti-venom and cytotoxic activity by in-vitro and in-vivo studies

Abstract

Snake bite is a serious problem in tropical and subtropical countries like India with 5 million human fatalities annually. The present study aimed on the shade grown Andrographis paniculata L under organic condition. 6 month old plants were collected at dawn; shade dried and extracted with methanol, followed by fractionation to isolate the active compound and characterization of the active compound was done using FT-IR study which was found to be identified as andrographolide. Furthermore, in vivo and in vitro studies confirm the anti- venom property of the plant and the cytotoxic levels determined by the use of cell lines has its major start towards the cellular level study. These studies and investigations confirms the presence of cobra venom inhibiting factor whose bite pose a huge threat to the life of people. Commercializing the drug against envenomation would prove successful in rural areas and the areas where the health centre is out of reach. Under economic circumstances, this study has the advantage of being very niggardly charged for the anti- venom preparation unlike the present day anti-venom that involves in rearing a horse and collecting the horse plasma that possess the raised anti- bodies which by further purification process would result in ASVS. Thus prepared ASVS holds the main disadvantage of bringing cross reactions like anaphylaxis and many more of the same sort. So, the plant as a drug would prove more effective, with high degree of safety and cost effective.