The effect of different concentration of epibrassinolide on chlorophyll, protein and anthocyanin content and peroxidase activity in excised red cabbage (brassica oleraceae l.) cotyledons

Abstract

Brassinosteroids are steroidal plant hormones that influence varied growth and development processes such as germination of seeds, flowering, senescence and abscission. This study aimed to explore the effect of cotyledon growth by different epibrassinolid (epiBL) concentrations in excised red cabbage cotyledons. As an experimental material, the cotyledons of 8-day-old Brassica oleraceae seedlings, excised from the petiole were incubated in 3 ml distilled water (control group) and in epiBL of various concentrations (0.001, 0.1 and 10 μM) for 3 days. Following incubation, peroxidase activity, total chlorophyll, soluble protein and anthocyanin contents were determined. The data indicate that the effect of epiBL on the growth of excised red cabbage cotyledons show difference related to concentration. © 2007 Taylor and Francis Group, LLC.