Chiral quantitation of pheniramine, chlorpheniramine, and brompheniramine maleates by capillary zone electrophoresis

Abstract

Cyclodcxtrin-mcdiated capillary zone electrophoresis is described for the simultaneous separation and quantitation of chiral pheniramine maleate, chlorpheniramine maleate, and brompheniramine maleate using scopolamine N-oxide as an internal standard. The chiral analysis of these drugs is performed in a phosphate buffer (75mM; pH 3.50) with β-cyclodextrin (10mM) and soluble anionic carboxymethyl β-cyclodextrin polymer (4 mg/mL) as chiral selectors. Several parameters affecting the separation are studied, including the type of chiral selector, the concentrations of phosphate buffer and chiral selector, and the pH of the buffer. The linear range of the method for the quantitation of these drugs is 25∼150μM for pheniramine maleate, chlorpheniramine maleate, or brompheniramine maleate, each as its enantiomer. This method is successfully applied to the assay of dexchlorpheniramine maleate in tablets.