Enhancement of pigment production potential of serratia marcescens (Gbb151) through mutation and random amplified polymorphic deoxyribonucleic acid analysis of its mutants

Abstract

Serratia marcescens (GBB151) was isolated and genetically modified for high-yielding pigment production capacity that could be employed for industrial purposes. Ethidium bromide-induced mutagenesis of GBB151 resulted in the generation of eight mutant isolates (GBB151Ea-GBB151Eh). The chemical mutants of S. marcescens obtained produced 5-fold more pigment than the wild-type organism. The wild-type GBB151 produced 413.9 unit/cell, while the mutant strains produced pigments with yields ranging from 841.7 to 2008.5 unit/cell. Random amplified polymorphic deoxyribonucleic acid-polymerase chain reaction analysis showed different amplicons patterns of native as well as mutant derivatives. The factorial analysis diagram and the dendrogram showed a degree of dissimilarity among the wild-type bacterial isolate GBB151 and its mutants. Mutant strains GBB151Ec and GBB151Ef were closest to the wild type as they appeared in the same quadrant. GBB151Ed which had lost its ability to produce pigment was farthest and in the different quadrant to the wild type. These study provided insight into improvement in pigment production by manipulating genetic make-up of S. marcescens, thus meeting industrial demand.