Comparing Diagnostic Protocols for Phytophthora ramorum in Rhododendron Leaves

Abstract

Phytophthora ramorum attacks many plant species important to the nursery industry and to the natural environment. USDA-APHIS requires all West Coast nursery stock to be inspected and, if necessary, tested for P. ramorum prior to export. The plants must be tested using a commercial DAS-ELISA kit and, if confirmed positive, the species-specific methods of PARP and PCR. This paper compares these diagnostic methods for the detection of P. ramorum in Rhododendron (L.) nursery stock.Accepted for publication 4 March 2005. Published 14 March 2005.