SNP Typing in Cereals: Comparison of SSCP and SNaPShot Markers Using the Barley Mlo Locus as a Model

Abstract

We compared the applicability of SSCP and SNaPShot markers in barley Mlo loci as a model of SNP detection in cereals. Whereas the development of SSCP markers required optimisation steps, the ddNTP primer extension (SNaPShot) procedure based on knowledge of target sequence provided expected results without any previous optimisations. We have shown, that SNPs can be easily scored using the ABI PRISM 310 Genetic Analyser.