Effects of melatonin implants on the reproductive performance and endocrine function of camel (Camelus dromedarius) bulls during the non-breeding and subsequent breeding seasons

Abstract

This study aimed to evaluate the effects of melatonin implants on the reproductive performances and hormone levels of dromedary (Camelus dromedarius) bulls during the non-breeding and subsequent breeding seasons. Fourteen mature dromedary bulls were divided into a control group (n = 7) and a group that was implanted with melatonin (n = 7) twice, at the beginning of the study and 35 days later. The trial started on the 17th June and continued for 10 weeks during the non-breeding season. Reproductive performances of animals in the control and melatonin groups were evaluated weekly during the non-breeding season [control non-breeding (CNB) and melatonin non-breeding (MNB) groups, respectively] and evaluated again during the subsequent breeding season [control breeding (CB) and melatonin breeding (MB) groups, respectively], which started on the 6th December and continued for 10 weeks. MNB bulls had greater (P ≤ 0.05) scrotum circumference and testicular volume, sexual desire, reaction time and mating ability scores, and serum melatonin and testosterone concentration values (24.91 ± 0.26 cm, 271.00 ± 7.81 cm3, 2.31 ± 0.13, 2.03 ± 0.22, 2.26 ± 0.09, 23.90 ± 0.05 pg/mL and 2764.51 ± 137.02 pg/mL, respectively) than the CNB group (23.63 ± 0.05 cm, 199.21 ± 3.27 cm3, 1.00 ± 0.00, 0.00 ± 0.00, 1.00 ± 0.00, 9.46 ± 0.08 pg/mL and 1872.41 ± 264.89 pg/mL, respectively). The scrotum and testes, reaction time score, proportion of bulls refusing to mount, and serum melatonin values were significantly higher in MB than CB bulls (P ≤ 0.05). Progressive motility (PM), average pathway velocity, straight-line velocity, curvilinear velocity (VCL), linearity, straightness (STR), wobble, beat cross frequency (BCF), livability, and DNA integrity were significantly higher in MB than CB bulls (P ≤ 0.05). PM, VCL, STR, amplitude of lateral head displacement, BCF, and livability were significantly higher in MNB than CB bulls (P ≤ 0.05). In conclusion, melatonin implants improved the reproductive performance of bulls during the non-breeding and subsequent breeding seasons.