Co-restoring multimodal microscopy images

Abstract

We propose a novel microscopy image restoration algorithm capable of co-restoring Phase Contrast and Differential Interference Contrast (DIC) microscopy images captured on the same cell dish simultaneously. Cells with different phase retardation and DIC gradient signals are restored into a single image without the halo artifact from phase contrast or pseudo 3D shadow-casting effect from DIC. The co-restoration integrates the advantages of two imaging modalities and overcomes the drawbacks in single-modal image restoration. Evaluated on a datasets of five hundred pairs of phase contrast and DIC images, the co-restoration demonstrates its effectiveness to greatly facilitate the cell image analysis tasks such as cell segmentation and classification.