Heparin inhibits phagocytosis by polymorphonuclear leukocytes

Abstract

Phagocytosis of unopsonized Salmonella typhimurium 395 MR-10, opsonized Salmonella typhimurium 395 MS, and Staphylococcus epidermidis by rabbit polymorphonuclear leuocytes was inhibited by heparin at concentrations as low as 0.5 U/ml. Inhibition was dose dependent and nearly complete at 20 U/ml. Provided that heparin concentrations did not exceed 100 U/ml, inhibition could be largely reversed by washing. Heparin also reversibly inhibited the adherence of polymorphonuclear leukocytes to glass. In contrast, hexose monophosphate shunt activity of polymorphonuclear leukocytes stimulated by noningested S. typhimurium MR-10 orStreptococcus pyogenes B14 was not inhibited by heparin at concentrations as high as 100 U/ml.