Electrochemical determination of vitamin B6 (pyridoxine) by reformed carbon paste electrode with iron oxide nanoparticles

Abstract

This work aims to introduce a simple high-performance sensitive electrochemical sensor of reformed carbon paste (CP) with iron oxide nanoparticles (IONs). It was developed to determine one of the most important drugs called vitamin B6 in a medium of phosphate buffer (PBS) over pH ranging from 5.0 to 8.0. The electrochemical and surface characterization was achieved by different techniques comprising electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), differential pulse voltammetry (DPV), and scanning electron microscopy (SEM). The studied effects were, pH, buffer, interference, scan rate, accumulation time, and calibration plot. The sensitive electrode produced a linear calibration curve in a concentration range from 8.88 up to 1000.0 µM, with limits of detection and qualification estimated at 9.06 and 30.2 µM, respectively Also, this method was established in human urine real samples and pharmaceutical drugs which have been shown a good result for vitamin B6 detection.