Abstract
A rapid identification of dengue viruses from clinical samples by using a nested reverse transcriptasepolymerase chain reaction (RT-PCR) procedure was carried out for diagnostic and epidemiological purposes. RT-PCR identified DEN-1 and DEN-2 viruses in 41% (41/100) of previously confirmed cases and provided an accurate confirmation of DHF in four fatal cases. RT-PCR was also useful for detecting and typing dengue viruses in suspected cases, allowing a rapid identification of new serotypes in endemic areas.
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Miagostovich, M. P., Dos Santos, F. B., De Araújo, E. S. M., Dias, J., Schatzmayr, H. G., & Nogueira, R. M. R. (1997). Diagnosis of Dengue by Using Reverse Transcriptase-Polymerase Chain Reaction. Memorias Do Instituto Oswaldo Cruz, 92(5), 595–600. https://doi.org/10.1590/S0074-02761997000500006
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