High Resolution Melting Analysis (HRM-PCR) – method and its application

Abstract

DNA denaturation with High Resolution Melting PCR-HRM is a method based on the identification of differences in the denaturation of PCR reaction products in the presence of fluorescent dyes. It is used to detect genetic variation in nucleic acid sequences in many branches of science, medicine and industry. This article is a review of the current literature of the methodology, applications and development of HRM analysis, which, thanks to its advantages such as speed, low cost, flexibility and simplicity, has found many applications, and its spectrum is still expanding.