Micro propagation and organogenesis of peperomia obtusifolia

Abstract

Peperomia obtusifolia is a bushy upright species belong to the Piperaceae, native to Florida, Mexico and the Caribbean, as an alternative to the conventional vegetative propagation methods is becoming an important way to increase shoot proliferation rates and produce of new varieties through transformation; therefore, the purpose of this study was to establish a protocol for in vitro production of Peperomia obtusifolia and to compare between two explants for shoot proliferation and organogenesis. Single node stem segments and leaf segments of Peperomia obtusifolia were cultured on solidified Murashige and Skoog (MS) basal medium supplemented with kinetin (Kin) at 0, 2.5, 5 and 10 mg L-1 and naphthalene acetic acid (NAA) at 0, 1, 2.5 and 5 mg L-1. All explants generated from leaf segments turned dark brown and died no shoots or callus were formed. Percentage of successful shoot regenerated from single node stem segments increased by increasing kinetin concentration then declined at the highest concentration of kinetin (10 mg L-1) while different concentrations of NAA almost had no effect on percentage of shoot formation. Kinetin at 5 mg L-1 combined with NAA at 1 mg L-1 was the most effective concentrations in percentage of shoot proliferation, number of shoots, fresh weight, total leaf area, total chlorophyll and callus weight on the other hand this concentration caused a reduction in shoot length and a delay in days to proliferation. © 2014 Asian Network for Scientific Information.