Carbon fixation of a temperate plankton community in response to calcium-and silicate-based Ocean Alkalinity Enhancement using air-sea gas exchange measurements

Abstract

Ocean Alkalinity Enhancement (OAE) is a carbon dioxide removal strategy that aims to chemically sequester atmospheric CO2 in the ocean while potentially alleviating localized effects of ocean acidification. Depending on the implementation approach, OAE can considerably alter seawater carbonate chemistry, resulting in temporarily reduced CO2 partial pressure (pCO2) and elevated pH before re-equilibration with the atmosphere or mixing with unperturbed waters. To investigate the effects of OAE on biogeochemical processes and organisms under close-To-natural conditions, a large-scale mesocosm experiment was conducted in a temperate fjord ecosystem near Bergen, Norway, during late spring. A non-CO2-equilibrated OAE approach was chosen, simulating OAE with calcium-and silicate-based minerals. A gradient of five OAE levels was achieved by increasing total alkalinity (TA) by 0-600 μmolkg-1. The added TA remained relatively stable over the 47 d experiment and measured CO2 gas exchange rates reached up to -15 mmol C m-2 d-1. We estimated that full equilibration (95 %) by air-sea gas exchange for a TA of 600 μmolkg-1 would take g1/41050 d. Furthermore, various mineral-Type and/or pCO2 / pH effects were found. Coccolithophore calcification followed an optimum curve response along the pCO2 gradient, consistent with findings from single-species laboratory cultures. In contrast, in-situ net community production (NCP) was higher in the silicate-based treatments, but was not modified by changes in pCO2. Zooplankton respiration, estimated from in-situ NCP and in-vitro NCP incubations, was lower for the silicate-based treatments and negatively correlated with pCO2. These complex findings suggest both direct and indirect effects of mineral type and OAE level and provide a valuable foundation for designing future OAE field trials. For a safe application of OAE, non-equilibrated alkalinity additions must balance efficiency and environmental impact.