Emerging of ptxP3 lineage in Bordetella pertussis strains circulating in a population in northeastern Mexico

4Citations
Citations of this article
16Readers
Mendeley users who have this article in their library.

Abstract

We determined the molecular epidemiology of Bordetella pertussis isolates to evaluate its potential impact on pertussis reemergence in a population of Mexico. Symptomatic and asymptomatic cases were included. Pertussis infection was confirmed by culture and real-time polymerase chain reaction (PCR). Selected B. pertussis isolates were further analysed; i.e. clonality was analysed by pulsed-field gel electrophoresis (PFGE) and ptxP-ptxA, prn, fim2 and fim3 typing was performed by PCR and sequencing. Out of 11 864 analysed samples, 687 (5.8%) were positive for pertussis, with 244 (36%) confirmed by both culture and PCR whereas 115 (17%) were positive only by culture and 328 (48%) were positive only by PCR. One predominant clone (clone A, n = 62/113; 55%) and three major subtypes (A1, A2 and A3) were identified by PFGE. All 113 selected isolates had the allelic combination ptxP3-ptxA1. The predominant clone A and the three major subtypes (A1, A2 and A3) corresponded to the emerging genotypes ptxP3-ptxA1-prn2-fim2-1-fim3-2 and ptxP3-ptxA1-prn2-fim2-1-fim3-1. In conclusion, the presence of an endemic clone and three predominant subtypes belonging to the genotypes ptxP3-ptxA1-prn2-fim2-1-fim3-2 and ptxP3-ptxA1-prn2-fim2-1-fim3-1 were detected. This finding supports the global spread/expansion reported for these outbreaks associated genotypes.

Cite

CITATION STYLE

APA

Gutiérrez-Ferman, J. L., Villarreal-Treviño, L., Ramírez-Aranda, J. M., Camacho-Ortiz, A., Ballesteros-Elizondo, M. R., Moreno-Juárez, M. R., … Garza-González, E. (2018). Emerging of ptxP3 lineage in Bordetella pertussis strains circulating in a population in northeastern Mexico. Epidemiology and Infection, 146(16), 2096–2101. https://doi.org/10.1017/S0950268818002303

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free