Probiotic research as highest antimicrobial Listeria monocytogenes from virgin coconut oil [VCO] Padang West Sumatra

Abstract

Virgin Coconut Oil [VCO] is a natural oil, made from fresh coconut milk fermentation without adding chemicals and other additives. Several Lactic acids Bacteria [LAB], have been isolate and characterization as probiotic. Several 5 different VCO sample products in local Padang Market have been determined. Specifics MRS medium has been used for LAB isolation and morphological characterization. The samples code are A, B, C, D, and E for 5 VCO samples. The highest total colony of LAB is from Samples A, which is about 1377 107 CFU/mL. 18 isolates have been identified as Gram-positive bacteria while 8 bacteria's are coccus and others form basil. All 18 LAB were grown at pH 2, and showing a resistance of about 49 %. Only 50 % of LAB resistant 0,3% oxgall for 4 hours. 3 isolate namely isolate A-1, A-3, and D-1 were showed the highest antimicrobial activities against Listeria monocytogenes with holozone averages of 26 mm. The hydrophobicity against xylene was also determined. The potential A-3 colonies are the highest percentages grown about 19% and resistant to several antibiotics such as Ampicillin, Chloramphenicol, Erythromycin, and Tetracycline. Molecular identification for potential A-3 using 16S RNA, showing phylogenetics species as Lactobacillus fermentum strain KF7.