Sequential ADP‐ribosylation pattern of nucleosomal histones: ADP‐ribosylation of nucleosomal histones

Abstract

The pattern of nucleosomal histones poly(ADP‐ribosyl)ation is changed under conditions which affect the poly(ADP‐ribosyl)ation state of the enzyme. At low NAD concentrations the enzyme can poly(ADP‐ribosyl)ate histones H1 and H1°, H2A, A2A, and H2B. However at NAD concentrations above 10 μM the enzyme preferentially poly(ADP‐ribosyl)ates histone H1 to a hyper ADP‐ribosylated form. Furthermore we have observed hyper ADP‐ribosylation of histone H2B at NAD concentrations of 10 μM suggesting that histone H2B can undergo the same type of ADP‐ribosylation pattern as histone H1. Also at higher NAD concentrations an elongation of the polymer attached to the enzyme and other nuclear proteins takes place. Copyright © 1985, Wiley Blackwell. All rights reserved