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Calcium phosphate transfection of primary hippocampal neurons.

Journal of visualized experiments : JoVE (2013) (81)
DOI: 10.3791/50808
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Abstract

Calcium phosphate precipitation is a convenient and economical method for transfection of cultured cells. With optimization, it is possible to use this method on hard-to-transfect cells like primary neurons. Here we describe our detailed protocol for calcium phosphate transfection of hippocampal neurons cocultured with astroglial cells.

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APA

Sun, M., Bernard, L. P., Dibona, V. L., Wu, Q., & Zhang, H. (2013). Calcium phosphate transfection of primary hippocampal neurons. Journal of Visualized Experiments : JoVE, (81). https://doi.org/10.3791/50808

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