Effects of growth regulators on in vitro response and multiple shoot induction in some endangered medicinal plants

Abstract

Introduction: The aim of this study was to focus on callus and multiple shoot induction in six endangered medicinal plants and subsequent media optimization for their potential com. applications. Materials and Methods: Here we used Cycas revoluta, Rauvolfia serpentina, Coleus barbatus, Tylophora indica, Ornithogalum spp. and Acorus calamus. Varying concns. of different plant growth hormones have been used for callus and shoot induction on Murashige and Skoog and B5 growth media. The auxins tested were IAA, NAA and 2,4-D. The cytokinins tested were benzylaminopurine (BAP) and kinetin. The explants chosen for tissue culture were based on their suitability and availability. For C. revoluta, both Murashige and Skoog and B5 have been used. Results: Explants of all the species showed the formation of multiple shoots. In Ornithogalum, multiple in vitro bulbs were formed. In case of C. revoluta (leaf explant), swelling was obsd. at 5 mg/L of 2,4-D in Murashige and Skoog media. Conclusion: Our observations are broadly consistent with previous tissue culture works on these plant species. This ensures possibility for further exploitation of these plants for probable micropropagation and plant regeneration on a com. scale. [on SciFinder(R)]