An Angiogenic Role for Adrenomedullin in Choroidal Neovascularization

7Citations
Citations of this article
16Readers
Mendeley users who have this article in their library.

Abstract

Purpose: Adrenomedullin (ADM) has been shown to take part in physiological and pathological angiogenesis. The purpose of this study was to investigate whether ADM signaling is involved in choroidal neovascularization (CNV) using a mouse model. Methods and Results: CNV was induced by laser photocoagulation in 8-week-old C57BL/6 mice. ADM mRNA expression significantly increased following treatment, peaking 4 days thereafter. The expression of ADM receptor (ADM-R) components (CRLR, RAMP2 and RAMP 3) was higher in CD31+CD45- endothelial cells (ECs) than CD31-CD45- non-ECs. Inflammatory stimulation upregulated the expression of ADM not only in cell lines but also in cells in primary cultures of the choroid/retinal pigment epithelium complex. Supernatants from TNFα-treated macrophage cell lines potentiated the proliferation of ECs and this was partially suppressed by an ADM antagonist, ADM (22-52). Intravitreous injection of ADM (22-52) or ADM neutralizing monoclonal antibody (mAb) after laser treatment significantly reduced the size of CNV compared with vehicle-treated controls (p<0.01). Conclusions: ADM signaling is involved in laser-induced CNV formation, because both an ADM antagonist and ADM mAb significantly inhibited it. Suppression of ADM signaling might be a valuable alternative treatment for CNV associated with age-related macular degeneration. © 2013 Sakimoto et al.

Cite

CITATION STYLE

APA

Sakimoto, S., Kidoya, H., Kamei, M., Naito, H., Yamakawa, D., Sakaguchi, H., … Takakura, N. (2013). An Angiogenic Role for Adrenomedullin in Choroidal Neovascularization. PLoS ONE, 8(3). https://doi.org/10.1371/journal.pone.0058096

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free