Expression and mutation analysis of Cyclin A and Ki‑67 in glioma and their correlation with tumor progression

Abstract

The present study aimed to analyze the expression and mutation of Cyclin A and Ki‑67 in gliomas, and determine their correlation with tumor progression. Tissue samples of 186 diagnosed glioma patients were examined immunohistochemically for Cyclin A and Ki‑67 expression. Gene mutation analysis was performed on genomic DNA extracted from patient samples, using polymerase chain reaction amplification and sequencing. Cyclin A and Ki‑67 expression were observed in the glioma and lymphatic metastasis tissues, and were analyzed using SPSS 14.0 statistical software. Of the total patients, 64 (34.41%) were Cyclin A‑positive and 68 (36.56%) were Ki‑67‑positive. The expression of Cyclin A and Ki‑67 in glioma was positively correlated with lymphatic metastasis. Statistically significant differences were observed in the mutation rate of Ki‑67 (P<0.05), but not Cyclin A (P>0.05), between the gliomas and metastatic tumors. In conclusion, Cyclin A and Ki‑67 are highly expressed in glioma tissues, and their expression and mutation are associated with the lymphatic metastasis of glioma in the brain. It may be concluded that Cyclin A and Ki‑67 may be used as biomarkers to guide the diagnosis of glioma and evaluate the prognosis of affected patients.