Comprehensive surveys of bean common mosaic virus and bean common mosaic necrosis virus and molecular evidence for occurrence of other phaseolus vulgaris viruses in tanzania

Abstract

Virus diseases are among the main biotic factors constraining common detected for the first time in common bean in Tanzania. Visually assessed bean (Phaseolus vulgaris L.) production in Tanzania. Disease manage-virus-like disease incidence ranged from 0 to 98% but reversement requires information on types, distribution, incidence, and genetic transcription polymerase chain reaction-based incidence of BCMV variation of the causal viruses, which is currently limited. Thus, a coun- and BCMNV (7,756 samples) was mostly less than 40%. The Sangertrywide comprehensive survey was conducted. Use of a next-generation based nucleotide sequences encoding coat proteins of BCMV and sequencing technique enabled simultaneous detection of 15 viruses be-BCMNV isolates were 90.2 to 100% and 97.1 to 100% identical to each longing to 11 genera. De novo assembly resulted in many contigs, includother, respectively. Phylogenetic analysis showed that BCMV isolates ing complete or nearly complete sequences of Bean common mosaic were more diverse than BCMNV isolates. The information generated virus (BCMV), Bean common mosaic necrosis virus (BCMNV), and in this study will contribute to the development of molecular diagnostic Southern bean mosaic virus (SBMV). Some viruses (for example, tools and strategies for management of virus diseases nationally and SBMV and Tomato leaf curl Uganda virus-related begomovirus) were internationally.