A cluster of ABA-regulated genes on Arabidopsis thaliana BAC T07M07

Abstract

Arabidopsis thaliana BAC T0M07 encoding the abscisic acid-insensitive 4 (ABI4) locus has been sequenced completely. It contains a 95,713-bp insert and 24 predicted genes. Most putative genes were confirmed by gel-based RNA profiling and a cluster of ABA-regulated genes was identified. One of the 24 genes, designated PP2C5, encodes a putative protein phosphatase 2C. The encoded protein was expressed in Escherchia coli, and its enzyme activity in vitro was confirmed.