Screening, evaluation of vibriocidal activity and characterization of bioactive substance from marine cyanobacteria

Abstract

The pathogens of Vibrio sp. mainly cause three diseases such as gastroenteritis, wound infection and septicaemia; they can also cause food borne infection. At present, marine cyanobacterial species such as Oscillatoria sp. and Lyngbya sp. were extracted using aqueous, ethyl acetate and pyridine extracts. The marine cyanobacterial extracts were evaluated for antivibrio activity by agar well diffusion method against Vibrio sp., such as Vibrio alginolyticus, Vibrio cholera and Vibrio fluvialis. Pyridine extract of Oscillatoria sp. shows maximum zone of inhibition and the crude extract was further separated by thin layer chromatography; and the separated fraction was evaluated for vibriocidal activity. The activated fraction was further analyzed by Fourier transforms infrared spectroscopy (FTIR) and the fraction showed the presence of phenols, aminoacids, sterols and saponins. The present study concludes that, the antivibrio metabolites of marine cyanobacteria are of special interest in the development of new harmless environment.