Design Of Autofocus Microscope With Histogram Method For Tuberculosis Bacteria Observation

Abstract

This research was conducted to design an autofocus microscope with a histogram method that can observe Tuberculosis (TB) bacteria. The bacteria observed were preparations or phlegm preparations which had been stained with Ziehl Neelsen. The microscope is designed to be equipped with a program to control the focus motor that moves the microscope tube and the program to digitally display the image and histogram of TB bacteria. Histograms are analyzed based on intensity values spread between 0-255 and the entropy value is sought. The measurement results that have been carried out as many as 20 times the field of view of the TB bacteria show that the most focused areas have the highest entropy value with an accuracy level ranging from 81.90476% to 100% at 1000 times the magnification.