IDENTIFIKASI DAN UJI RESISTENSI Pseudomonas sp. TERHADAP ANTIBIOTIK GENTAMISIN, KLORAMFENIKOL DAN SIPROFLOKSASIN PADA DAGING SAPI DI PASAR TRADISIONAL KOTA KUPANG

Abstract

Beef is a food source that contains essential nutrients for humans. The high nutrient and water content provide a favorable environment for bacteria like Pseudomonas sp. This study purpose is to detect the existence of Pseudomonas sp. and determine its resistance to gentamicin, chloramphenicol, and ciprofloxacin. Purposive sampling used to collect 12 beef samples from three Kupang City marketplaces, which were then isolated, identified, and tested for antibiotic resistance. The study's results revealed the presence of Pseudomonas sp. bacteria in meat samples from these marketplaces. The antibiotic resistance test findings showed that 16.66% of Pseudomonas sp. bacteria were sensitive to gentamicin, 83.3% were intermediate, 33.33% were sensitive to chloramphenicol, 66.66% were intermediate, and 100% were intermediate to ciprofloxacin. The study's findings are projected to serve as the foundation for policy decisions about food supply management in the Kupang City area that adhere to the Safe, Healthy, Whole, and Halal standards.