REG I enhances chemo- and radiosensitivity in squamous cell esophageal cancer cells

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Abstract

Identification of reliable markers of chemo- and radiosensitivity and the key molecules that enhance the susceptibility of squamous esophageal cancer cells to anticancer treatments would be highly desirable. To test whether regenerating gene (REG) I expression enhances chemo- and radiosensitivity in esophageal squamous cell carcinoma cells, we used MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assays to compare the chemo- and radiosensitivities of untransfected TE-5 and TE-9 cells with those of cells stably transfected with REG Iα and Iβ. We then used flow cytometry to determine whether REG I expression alters cell cycle progression. No REG I mRNA or protein were detected in untransfected TE-5 and TE-9 cells. Transfection with REG Iα and Iβ led to strong expression of both REG I mRNA and protein in TE-5 and TE-9 cells, which in turn led to significant increases in both chemo- and radiosensitivity. Cell cycle progression was unaffected by REG I expression. REG I thus appears to enhance the chemo- and radiosensitivity of squamous esophageal cancer cells, which suggests that it may be a useful target for improved and more individualized treatments for patients with esophageal squamous cell carcinoma. © 2008 Japanese Cancer Association.

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Hayashi, K., Motoyama, S., Koyota, S., Koizumi, Y., Wang, J., Takasawa, S., … Sugiyama, T. (2008). REG I enhances chemo- and radiosensitivity in squamous cell esophageal cancer cells. Cancer Science, 99(12), 2491–2495. https://doi.org/10.1111/j.1349-7006.2008.00980.x

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