GC-MS ANALYSIS OF PHYTOCHEMICALS IN THE METHANOLIC EXTRACT OF Caesalpinia coriaria (Jacq) Willd.

Abstract

Background: The presence of diverse secondary metabolites (phytochems.) has been reported from the genus Caesalpinia. However, there has been not much information available on phytochem. components and biol. activity in the methanol ext. of Caesalpinia coriaria (Jacq) Willd. Objective: This study was designed to det. the phytochems. in the methanol ext. of Caesalpinia coriaria (Jacq) Willd. Materials and Methods: Using GC-MS the mol. mass of a compd. and its elemental compn. could be easily detd. High resoln. Electron Impact Mass Spectroscopy was performed. For GC-MS anal. a 30 × 0.32 mm × 1.8 μm column Shimadzu QP2012 was used. For GC-MS detection an electron system with ionization energy of -70eV was used. Helium gas (99.9995%) was used as the carrier gas at a const. flow rate of 1.491mL/min and an injection vol. of 1.0ml was employed with split ratio 10:1. Injector temp. 140° C Ion-source temp. 200°C. The oven temp. was programmed from 110° with an increase of 10°C/min to 240°C ending with 8 min. Mass spectra were taken at 70eV a scan range of 0.5 s. Total GC running time was 24.35 min. The relative percentage amt. of each component was calcd. by comparing its av. peak area to the total areas. Software adopted to handle mass spectra and chromatograph was GCMS soln. version 2.53 and compared with NIST Library 2011 version. The name, mol. wt. and the structure of the components of the test material were ascertained. GC-MS anal. of the ethanol ext. of Caesalpinia coriaria was performed using a GC-MS-5975C [AGILENT] comprising an HP-5ms Agilent auto-sampler and a gas chromatograph interfaced to a mass spectrometer (GC-MS). Results: This investigation was carried out to det. the possible chem. components from Caesalpinia coriaria by GC-MS. This anal. revealed that the methanol ext. of Caesalpinia coriaria contained of 12 compds. Dodecadien-1-ol, n-Hexadecanoic acid Tridecanoic acid Hexadecanoic acid were present abundantly. These compds. have pesticide, nematicide, acidifier, antitumor activity. 3-Cyclopentylpropionamide, 2 Et 9,12,15-octadecatrienoate-Octadecatrienoic acid and Vitamin E have Antidote, antitumor Antiproliferative Hypercholesterolemic, antiarthritic, antiacne, hepatoprotective Antidote, anticancer activity. All identified compds. were, generally, reported as having antimicrobial activity. In addn., the squalene compd. also having anti-cancer, anti-oxidant, anti-tumor, chemo-preventive, pesticidal. Conclusions: From the results, it is evident that C. coriaria contains various bioactive compds. and is recommended as a plant of phytopharmaceutical importance. [on SciFinder(R)]