Segregation of T-DNA inserts in the offspring of Arabidopsis thaliana after Agrobacterium transformation

Abstract

Using various transformation methods, T-DNA constructions for insertional mutagenesis were introduced into Arabidopsis thaliana and the pattern of segregation of hygromycin resistance selectable marker was followed in succeeding generations in individual transgenic lines up to T4 generation. Despite the low frequency of transformation, T-DNA was often inserted in two or more independent sites. Mendelian segregation ratios 3:1, 15:1, and irregular segregation ratios were observed. We have also shown continuous decrease of the expression of the resident hygromycin resistance transgenic trait in some lines.